Xiao-Yue Deng, Hu Wang, Tao Wang, Xian-Tao Fang, Li-Li Zou, Zhi-Ying Li and Chang-Bai Liu Pages 153 - 158 ( 6 )
Induced pluripotent stem (iPS) cells were created from mouse fibroblasts by induced expression of Yamanaka factors, Oct3/4, Sox2, Klf4, and c-Myc. This technique has quickly resulted in an exponential increase in the amount of pluripotency studies, and has provided a valuable tool in regenerative medicine. At the same time, many methodologies to generate iPS cells have been reported, and are comprised mainly of viral methods and non-viral methods. Although viral methods may not be applicable for clinical applications, various nonviral methods have been reported in recent years, including DNA vector-based approaches, transfection of mRNA, transduction of reprogramming proteins, and use of small molecule compounds. This review summarizes and evaluates these non-viral methods.
DNA integration-free, induced pluripotent stem cells, non-viral methods.
Institute of Molecular Biology, China Three Gorges University, 8 Daxuelu, Yichang, China.