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Comparison of Isolation, Expansion and Cryopreservation Techniques to Produce Stem Cells from Human Exfoliated Deciduous Teeth (SHED) with Better Regenerative Potential

Author(s):

Sau Har Lee*, Chung Yeng Looi, Pei Pei Chong, Jhi Biau Foo, Qi Hao Looi, Chu Xin Ng and Zaidah Ibrahim   Pages 1 - 12 ( 12 )

Abstract:


Mesenchymal stem cells (MSCs) are adult stem cells that are gaining worldwide attention for its multi-potential use in tissue engineering-based regenerative medicine. They can be obtained from numerous sources and one of the excellent source is from the dental tissue, such as stem cells that are extracted from the human exfoliated deciduous teeth (SHEDs). SHEDs are considered ideal due to its inherent characteristics, including the capability to proliferate quickly with minimal oncogenesis risk, multipotency capacity and its ability to suppress the immune system. On top of these positive cell traits, SHEDs are easily accessible with patient’s safety assured, posing less ethical issues and could also provide sufficient cells number for prospective clinical uses. This is primarily attributed to its ability to differentiate into multiple cell linages, including osteoblasts, odontoblasts, neuronal cells, adipocytes, as well as endothelial cells. Albeit SHEDs having a bright future, there still remains an obstacle to develop reliable experimental techniques to retain the long term regeneration potential of the stem cells for prospective research and clinical applications. Therefore, this review aims to describe the various isolation, expansion and cryopreservation techniques used by researchers in this stem cell field. Optimization of these techniques is crucial to obtain distinct SHEDs culture with preserved stem cells properties, which enable more reproducible results that will be the key for further stem cells therapy development.

Keywords:

Human exfoliated deciduous teeth (SHED), Isolation technique, Expansion technique, Cryopreservation technique, Mesenchymal stem cells (MSCs), Regenerative potential

Affiliation:

School of Biosciences, Faculty of Health and Medical Sciences, Taylor’s University, Lakeside Campus, 47500 Subang Jaya, Selangor Darul Ehsan, School of Biosciences, Faculty of Health and Medical Sciences, Taylor’s University, Lakeside Campus, 47500 Subang Jaya, Selangor Darul Ehsan, School of Biosciences, Faculty of Health and Medical Sciences, Taylor’s University, Lakeside Campus, 47500 Subang Jaya, Selangor Darul Ehsan, School of Pharmacy, Faculty of Health and Medical Sciences, Taylor’s University, Lakeside Campus, 47500 Subang Jaya, Selangor Darul Ehsan, Regena Asia Sdn Bhd, No 37, Lorong Bukit Gambir 2, Ashley Green, 11700 Gelugor, Pulau Pinang, School of Biosciences, Faculty of Health and Medical Sciences, Taylor’s University, Lakeside Campus, 47500 Subang Jaya, Selangor Darul Ehsan, School of Biosciences, Faculty of Health and Medical Sciences, Taylor’s University, Lakeside Campus, 47500 Subang Jaya, Selangor Darul Ehsan



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