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Mini-Review; Deriving Avian Stem Cells by Small Molecules

Author(s):

Maryam Farzaneh*   Pages 1 - 5 ( 5 )

Abstract:


Avian embryos and related cell lines have found wide applications in basic and applied sciences. The embryonated egg is a great host for monoclonal antibodies and recombinant proteins. Avian celllines derived from embryonated eggs have been used for the product ion of transgenic birds and virusinoculationin order tovaccine preparation. Hitherto, many efforts have been invested to develop efficient avian stem cell culture. Under the conventional conditions, there are various challenges such as the type of feeder layers, conditioned medium,serum, and growth factors. Researchers have investigated different conditions to solve these problems. Recent studies have shown that targetedstrategiesusingsmallmoleculeinhibitorscouldbeusedasalternativestomulti-growth factor delivery approaches. Since small molecule inhibitors were used for mammalian pluripotent stem cells (PSC), several kinds of research have examined the effect of small molecule on self-renew a land maintenance of avian PSC. Avian PSC can be derived from early blastodermal cells (stage X), circular primoridial germ cells (PGC; stage HH17), gonadal PGC (stage HH28), and embryonic germ cells (EGC; HH28).Previous studies have shown that the use of small molecule drugs such as PD0325901,SB431542,SC1,IDE1,Z-VAD, Blebbistatin,H-1152,and IDE1 could bean efficient method for the derivation of avian stem cells. This mini-review covers there cent development of avian stem cell culture by small molecules.

Keywords:

Stem cells, avian pluripotent stem cells, small molecules, recombinantproteins, transgenicbirds, vaccine.

Affiliation:

Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz



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