Elham Sabouri, Alireza Rajabzadeh, Seyedeh Elnaz Enderami, Ehsan Saburi, Fatemeh Soleimanifar, Ghasem Barati, Mohamad Rahmati, Gholamreza Khamisipour* and Seyed Ehsan Enderami* Pages 141 - 150 ( 10 )
Stem cell-based therapy is one of the therapeutic options with promising results in the treatment of diabetes. Stem cells from various sources are expanded and induced to generate the cells capable of secreting insulin. These insulin-producing cells [IPCs] could be used as an alternative to islets in the treatment of patients with diabetes. Soluble growth factors, small molecules, geneencoding transcription factors, and microRNAs [miRNAs] are commonly used for the induction of stem cell differentiation. MiRNAs are small non-coding RNAs with 21-23 nucleotides that are involved in the regulation of gene expression by targeting multiple mRNA targets. Studies have shown the dynamic expression of miRNAs during pancreatic development and stem cell differentiation. MiR- 7 and miR-375 are the most abundant miRNAs in pancreatic islet cells and play key roles in pancreatic development as well as islet cell functions. Some studies have tried to use these small RNAs for the induction of pancreatic differentiation. This review focuses on the miRNAs used in the induction of stem cells into IPCs and discusses their functions in pancreatic β-cells.
Diabetes, stem cells, pancreatic differentiation, MicroRNAs, insulin-producing cells, islet-like clusters
Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Applied Cell Sciences and Tissue Engineering Department, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Department of Stem Cell and Regenerative Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology [NIGEB], Tehran, Medical Genetics and Molecular Medicine Department, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Department of Medical Biotechnology, School of Medicine, Alborz University of Medical Sciences, Karaj, Stem Cell Technology Research Center, Tehran, Stem Cell Technology Research Center, Tehran, Department of Hematology, School of Allied Medical Sciences, Bushehr University of Medical Sciences, Bushehr, Diabetes Research Center, Department of Medical Biotechnology, Faculty of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari