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Transplantation of Adipose Tissue-Derived Stem Cells into Brain Through Cerebrospinal Fluid in Rat Models: Protocol Development and Initial Outcome Data

[ Vol. 14 , Issue. 2 ]

Author(s):

Naser Amini, Nasim Vousooghi, Akram Alizade, Sara Ramezani, Mohammad T. Joghataei*, Peiman Brouki Milan, Soraya Mehrabi, Shima Ababzadeh, Farshid Sefat and Masoud Mozafari*   Pages 191 - 195 ( 5 )

Abstract:


Background: Cell therapy is an important strategy for the treatment of incurable diseases including those that occur in the Central Nervous System (CNS). Among different strategies, the method of delivering or transplantation of cells into the brain has shown significant effects on regeneration. In this study, a new protocol has been developed for the transplantation of adipose tissuederived stem cells into the brain through Cerebrospinal Fluid (CSF) in rat models.

Methods: For this purpose, a wide range of ages (7-30 days old) of male neonates of Wistar rats was used. Moreover, human adipose tissue was obtained from a superficial layer of abdomen through liposuction surgery. The size of the inserted part of needle to access middle cranial fossa and subarachnoid space in animals with an average weight of 10-80 g was determined. In addition, to confirm the entrance of needle into the subarachnoid space, CSF was aspirated slowly and then injection was done within two minutes.

Results: The findings showed the presence of transplanted human Adipose-Derived Stem Cells (hADSC) in the cerebellum and basal ganglia following three days and also after two months that confirmed the entrance of transplanted cells into the cerebrospinal fluid and migration of them into the brain tissue. All the animals survived after the transplantation process, with the lowest side effects compared to the available conventional methods.

Conclusion: It can be concluded that the cells could be efficiently transplanted into CSF through subarachnoid space by injection via superior orbital fissure with a minimally invasive technique.

Keywords:

Nervous system, transplantation, stem cell, cerebrospinal fluid, brain tissue, adipose tissue.

Affiliation:

Cellular and Molecular Research center, Iran University of Medical Sciences, Tehran, Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Neuroscience Research Center, School of Medicine, Guilan University of Medical Sciences, Tehran, Cellular and Molecular Research center, Iran University of Medical Sciences, Tehran, Cellular and Molecular Research center, Iran University of Medical Sciences, Tehran, Cellular and Molecular Research center, Iran University of Medical Sciences, Tehran, Qom University of Medical Sciences, Qom, Biomedical Engineering and Electronics Department, School of Engineering and informatics, University of Bradford, Bradford, Cellular and Molecular Research center, Iran University of Medical Sciences, Tehran



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