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Future of Spermatogonial Stem Cell Culture: Application of Nanofiber Scaffolds

[ Vol. 12 , Issue. 7 ]

Author(s):

Azar Shams , Neda Eslahi , Mansoureh Movahedin, Fariborz Izadyar, Hamidreza Asgari and Morteza Koruji   Pages 544 - 553 ( 10 )

Abstract:


Background: Spermatogonial stem cells (SSCs) are unique in mammals because they can transmit genetic information from generation to generation and it is of significant importance. In testes, Sertoli cells, peritubular myoid cells, Leydig cells and other interstitial cells contribute to the spermatogonial stem cell “niche”. So, creation of niche in an in vitro condition that mimics the in vivo environment is essential to maintain functional characteristic of SSCs.

Objective: In this review, we describe the impact of nanofiber scaffolds on the culture of SSCs derived from human-to-mouse.

Results: Nanofiber Matrices mimic the architecture and size scale of the natural extracellular matrix (ECM). The scaffold provides more three-dimensional (3D), biomimicking and topographical signals to the cells and results in a more physiologically relevant cellular phenotype. Several investigators use different nanofiber scaffold-like carbon nanotubes (CNTs) scaffold, poly-L-lactic acid (PLLA) nanofiber scaffold, 3D soft agar culture system, human serum albumin (HSA)/tri calcium phosphate nanoparticles (TCPNPs) and electrospun polyamide nanofiber for proliferation and maintenance of self-renewal activity of the SSCs.

Conclusion: Application of nanofiber scaffolds for in vitro culture of the SSCs may produce spermatogonial stem cells that can be used in regenerative medicine, tissue engineering, assisted reproductive technology and in the treatment of infertility in pre-pubertal cancer patients.

Keywords:

Nanofiber, spermatogonial stem cells, culture system, scaffolds, tissue engineering.

Affiliation:

Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Anatomical Sciences Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, PrimeGen Biotech LLC, 2917 Daimler St. Santa Ana CA 92705, Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Sciences, Iran University of Medical Sciences (IUMS), Hemmat Highway, P. O. Box 14155-5983, Tehran



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