Cristian Nunez-Espinosa, Ines Ferreira, Juan Gabriel Rios-Kristjansson, David Rizo-Roca, Maria Dolors Garcia Godoy, Laura G. Rico, Gerard Rubi-Sans, Joan Ramon Torrella, Teresa Pages, Jordi Petriz and Gines Viscor Pages 132 - 139 ( 8 )
Our goal was to address if intermittent hypobaric hypoxia (IHH) exposure can help to increase the number of peripheral blood circulating progenitor cells and side population (SP) stem cells, in order to establish the usefulness of this intervention for skeletal muscle repair, because these cells play a role in tissue regeneration. Male Sprague-Dawley rats were studied in two basal states: untrained and trained and compared with 1, 3, 7 and 14 days stages of damage recovery of trained rats that had suffered skeletal muscle injury. Three experimental groups were studied: rats with passive recovery (CTRL); rats exposed to IHH after muscle damage (HYP); and, trained rats that, in addition to IHH, performed light aerobic exercise sessions (EHYP). We observed an increase in hematopoietic stem cells (HSCs) (mean = 0.153% of cells) and endothelial progenitor cells (EPCs) (mean = 0.0020 % of cells) in EHYP on day 7. Also these cells showed characteristics of more primitive progenitors in comparison to the other experimental groups (mean = 0.107 % of cells), as deduced by retention of the promising fluorescent probe Vybrant Dye Cycle Violet. We concluded that intermittent exposure to hypobaric hypoxia in combination with light aerobic exercise increased the number of HSCs and EPCs on the 7th day in EHYP group, although the exercise-induced stimulus showed a reverse effect on SP kinetics.
Eccentric exercise, intermittent hypobaric hypoxia, side population cells, stem cells.
Departament de Fisiologia i Immunologia, Universitat de Barcelona, Av. Diagonal, 643; E-08028 Barcelona, Spain.